The aim of the study was to investigate the hepatoprotective effects of Folium syringae (FS) extracts against ethanol-induced acute liver injury.Mice and primary hepatocytes were pretreated with FS extracts at different dosages before ethanol administration.Transaminases,glutathione S-transferase A1 level and hepatic biochemical indices (malondialdehyde,superoxide dismutase,glutathione and glutathione peroxidase) were determined.Pretreatment with FS extracts significantly inhibited the damage caused by ethanol and the hepatoprotective effects of FS were almost similar to Silymarin that was used to treat alcoholic liver injury.GSTA1 contents in all the FS extract-treated groups were significantly different from those in the ethanol-induced acute liver injury model group (p＜0.01),and similar trends were observed in transaminases and hepatic indices level both in vitro and in vivo.The results showed that FS extracts had hepatoprotective effects against ethanol-induced injury.Those effects might be related to the enhancement of antioxidant capacity of liver cells,and FS extracts could reduce the release of liver GSTA1,which contributed to improve liver detoxification.