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目的:观察阿米洛利(Am)与丹参酮ⅡA(Tsn)联用预防大鼠肝纤维化的疗效。方法;建立二甲基亚硝胺大鼠肝纤维化模型,观察Am与Tsn预防后,肝／体和脾／体比值、肝功能、羟脯氨酸、透明质酸及层粘连蛋白含量变化。结果:除Am预防组外,Tsn预防组及Am加Tsn预防组较模型组肝功能得到改善(P<0.01);Am和Tsn预防组较模型组血清透明质酸、层粘连蛋白和肝组织羟脯氨酸含量降低(P<0.01);预防组肝组织内超氧化物歧化酶、谷胱甘肽过氧化物酶较模型组升高,而丙二醛明显低于模型组(P<0.01)。结论:Am与Tsn对二甲基亚硝胺诱导的肝纤维化均有预防作用,两者合用有一定的协同作用。
Objective: To observe the effect of amiloride (Am) combined with tanshinone ⅡA (Tsn) in preventing hepatic fibrosis in rats. Methods The rat model of hepatic fibrosis induced by dimethylnitrosamine was established. The changes of liver / body and spleen / body ratio, liver function, hydroxyproline, hyaluronic acid and laminin were observed after Am and Tsn prophylaxis. Results: In addition to the Am group, the hepatic function of Tsn prophylaxis group and Am plus Tsn prophylaxis group was improved compared with the model group (P <0.01). The levels of serum hyaluronic acid, laminin and hepatic tissue hydroxyproline (P <0.01). The levels of superoxide dismutase and glutathione peroxidase in liver tissue of prophylaxis group were higher than those of the model group, while the content of malondialdehyde was significantly lower than that of the model group (P <0.01) . Conclusion: Both Am and Tsn have preventive effects on DMN induced by dimethylnitrosamine, and there is certain synergistic effect between them.