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转基因山羊体细胞克隆羊诞生首例转基因试管牛问世中科院发育生物学研究所、扬州大学利用转基因山羊体细胞克隆两头羔羊。该研究组从一头黑母羊卵巢采集颗粒细胞,细胞在体外培养和处理后移入白山羊的去核卵母细胞,经融合、激活后包埋在琼脂块内,在寄母羊(白色)输卵管内培养,最后把发育的囊胚移入白山羊母羊子宫内,最后分娩出两头羔羊。经 DNA 指纹检测,确证这两头羔羊是来自成年转基因母羊体细胞的克隆羊。这是该研究组继1991年利
Transgenic goat somatic cloned sheep was born The first case of transgenic cow was born Institute of Developmental Biology, Chinese Academy of Sciences, Yangzhou University, somatic cell cloning using lambs. The research group collected granulosa cells from a black ewes ovary. The cells were cultured and treated in vitro and then transferred into enucleated oocytes of white goats. After fusion and activation, the cells were embedded in agar blocks. In the end, the development of the blastocyst moved into the white goat ewe womb, and finally gave birth to two lambs. DNA fingerprinting confirmed that the two lambs were cloned sheep from adult transgenic ewes. This is the research group following 1991 Lee