组织学已证明在胃粘膜上存在的幽门弯曲菌与胃炎和消化性溃疡密切相关。但这种微生物的致病机理还不清楚。因为大多数细菌的毒力都是由质体介导的,为此作者决定检查分离的幽门弯曲菌的质体。通过胃镜观察,从患有上消化道疾病患者的胃和十二指肠活检标本上取得幽门弯曲菌并进行分离,经公认的标准进行鉴定。采用改良的Lastovica和Ambrosio方法提取质体DNA。把质体DNA在0.6％的水平琼脂糖胶上以36V电压电泳18小时。用溴化乙锭1μg/ml染色,在300nm处观测。 Histology has shown that the presence of Campylobacter pylori in the gastric mucosa is closely related to gastritis and peptic ulcer. However, the pathogenesis of this microorganism is unclear. Because most bacterial virulence is mediated by plastids, the authors decided to examine the plastid of the isolated H. pylori. Campylobacter pylori was isolated from stomach and duodenal biopsies from patients with upper gastrointestinal diseases by gastroscopy and was identified by accepted standards. Plasmid DNA was extracted using the modified Lastovica and Ambrosio methods. The plastid DNA was electrophoresed on a 0.6% agarose gel at a voltage of 36V for 18 hours. Stained with ethidium bromide 1 μg / ml and observed at 300 nm.