目的 :研究非病毒载体法基因转染的最适条件及其潜在价值。方法 :利用脂质体介导和配体定向法将带有野生型 P5 3 基因 ( Wt- P5 3 ) c DNA的质粒引入细胞 ,用直接镜检、软琼脂培养、四甲基偶氮唑 ( MTT)法和乳酸脱氢酶法等研究细胞生长特性的改变情况。结果 :绝大多数细胞被转染 ,肿瘤细胞生长受到明显抑制而发生凋亡。结论 :本方法对于基因体外转染是可行的 ,并且有可能将来用于小细胞肺癌的 P5 3 基因治疗 Objective: To study the optimal conditions and potential value of non-viral vector gene transfection. Methods: Plasmid with wild-type P5 3 gene (Wt-P5 3) c DNA was introduced into the cells by liposome-mediated and ligand-directed method. The cells were stained with direct microscopy, soft agar, MTT) method and lactate dehydrogenase method to study the changes of cell growth characteristics. Results: The vast majority of cells were transfected, tumor cells were significantly inhibited apoptosis. CONCLUSIONS: This method is feasible for gene transfection in vitro and is likely to be used for future P5 3 gene therapy in small cell lung cancer