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用液体法培养了小鼠的GFU—GM,并对培养不同时间的集落细胞进行扫描电镜观察。CFU—GM在分化成熟过程中有如下特点:(1)粒系细胞集落细胞表面由粗糙变光滑,主要变化在其表面的微绒毛。(2)单系细胞集落在培养早期成团,细胞表面光滑,4天后变粗糙,形状由规则变成多形性,由大小均匀至大小不一;培养中期有伪足形成.(3)同一集落位于中心的细胞较周边细胞幼稚.同时还发现粒细胞集落基底多存在一个贴壁细胞.
The GFU-GM of mice was cultured by liquid method, and the colony cells cultured for different time were observed by scanning electron microscopy. CFU-GM in the process of differentiation and maturation has the following characteristics: (1) Granulocyte colony cell surface from rough to smooth, the main changes in its surface microvilli. (2) The mononuclear cell colonies formed into clusters in the early stage of culture, the cell surface became smooth, and became rough after 4 days. The shape changed from regular to pleomorphic, from uniform size to different size; pseudopodia formed in the middle of culture. (3) The cells in the center of the colony were naive compared with the surrounding cells, and there was also one more adherent cell in the colony base.