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牛与人的CuZn-超氧化物歧化酶(简称CuZn-SOD)虽为不同种属的酶,但其一级结构中150个氨基酸残基中有125个排列顺序相同,而且两者的二、三、四级结构也可能类似。如果肿瘤患者血中CuZn-SOD的结构发生些微变化,是否会改变该酶与抗牛CuZn-SOD的抗血清之间抗原抗体反应的强不相容性,至今无人研究。为了研究此问题,我们建立了某些方法与新技术如(1) 分离纯化牛CuZn-SOD。其比活性与紫外吸收、电泳图谱等理化性质完全达到纯酶标准。(2) 制备了抗牛CuZn-SOD的抗血清。用免疫双扩散法鉴定出抗血清效价为1∶32。抗血清可与有活性或无活性的牛CuZn-SOD发生强抗原抗体反应。(3) 建立了激光比浊的抗原抗体复合物定量法。该法手续简便,灵敏度较高。(4) 新建立了化学发光的抗原抗体复合物定量法,其特点是应用我们推导的动力学公式
Although CuZn-SOD is a kind of enzyme of different species, CuI-SOD of cow and human have the same order of 125 in the 150 amino acid residues in the primary structure, Three or four levels of structure may be similar. If there is a slight change in the structure of CuZn-SOD in the blood of tumor patients, whether it will change the strong incompatibility of the antigen and antibody reaction between the enzyme and the anti-bovine CuZn-SOD antiserum has not been studied so far. In order to study this problem, we established some methods and new technologies such as (1) isolation and purification of bovine CuZn-SOD. The specific activity and UV absorption, electrophoresis and other physical and chemical properties of pure enzyme standards. (2) Antiserum against cattle CuZn-SOD was prepared. Antiserum was identified as 1:32 by double immunodiffusion. Antiserum can react with strong antigen and antibody with active or inactive bovine CuZn-SOD. (3) Established a laser turbid antigen-antibody complex quantitative method. The procedure is simple, high sensitivity. (4) The newly established chemiluminescence antigen-antibody complex quantification method, which is characterized by our derived kinetic formula