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目的:检查左旋氧氟沙星在体外的抗分支杆菌活性。设计:用7H11培养基,以琼脂稀释方法测左旋氧氟沙星对不同种的分支杆菌的最小抑制浓度(MIC),并与氧氟沙星比较。左旋氧氟沙星对于被小鼠腹腔巨噬细胞吞食的结核分支杆菌和胞内分支杆菌的抗微生物活性是以菌落形成单位(CFU)的减少来衡量的。结果:左旋氧氟沙星抗结核分支杆菌、堪萨斯分支杆菌、海鱼分支杆菌、瘰疬分支杆菌、鸟分支杆菌、胞内分支杆菌、偶然分支杆菌和龟分支杆菌的MIC比氧氟沙星的MIC低2到4倍。左旋氧氟沙星减少巨噬细胞内CFU的效果高于氧氟沙星。结论:左旋氧氟沙星比氧氟沙星具有更强的体外抗分支杆菌活性。
OBJECTIVE: To examine antimycobacterial levofloxacin activity in vitro. Design: The minimum inhibitory concentration (MIC) of levofloxacin against different mycobacteria was determined by agar dilution method using 7H11 medium and compared with ofloxacin. Anti-microbial activity of levofloxacin against Mycobacterium tuberculosis and M. intracellulare phagocytosed by mouse peritoneal macrophages was measured as a decrease in colony-forming units (CFU). Results: MICs of Levofloxacin against Mycobacterium tuberculosis, Kansas mycobacterium, Mycobacterium maritima, Mycobacterium phlei, Mycobacterium avium, M. intracellulare, M. fortuitum and M. turtle were MICs higher than those of ofloxacin 2 to 4 times lower. Levofloxacin to reduce the effect of CFU macrophages than ofloxacin. CONCLUSION: Levofloxacin has stronger in vitro antimycobacterial activity than ofloxacin.