已糖醛酸和已糖胺是软骨基质中糖胺多糖的主要成份,测定二者的含量以及它们的比例关系,对判定软骨的性质及糖胺多糖的性质是十分重要的。但是已糖醛酸在测化过程中容易遭受破坏,使测定值大大降低。用测定已糖胺的消化条件消化软骨,根本就不能用Bitter等改良的咔唑反应测定已糖醛 The uronic acid and hexosamine have been the main components of the glycosaminoglycans in the cartilage matrix. It is very important to determine the content of the glycosaminoglycans in the cartilage matrix and their ratios, and to determine the nature of the cartilage and the properties of the glycosaminoglycans. However, uronic acid has been easily destroyed during the measurement process, and the measured value is greatly reduced. The cartilage is digested with the glycosaminoglycan digestion condition, and the modified carbazole reaction by Bitter et al.