【摘 要】
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ABSTRACT Objective:To evaluate parasite distribution and tissue tropism ofToxoplasma gondii tachyzoites in experimentally infected mice using real timeQPCR.Meth
【机 构】
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Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran“,”Molecular and
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ABSTRACT Objective:To evaluate parasite distribution and tissue tropism ofToxoplasma gondii tachyzoites in experimentally infected mice using real timeQPCR.Methods:In this survey16Balb/c mice were inoculated with1×104 alive tachyzoites ofToxoplasma gondiiRH strain.After1,2,3 days post infection and the last day(before death), different tissues of mice including blood, brain, eye, liver, spleen, kidney, heart and muscle were harvested.Following tissuesDNA extraction, the parasite burden was quantified using real timeQPCR targeting theB1 gene(451 bp).Results:It showed thatToxoplasmaafter intraperitoneal injection was able to movement to various tissues in 24 hours.Parasite burden was high in all tissues but the most number of parasites were observed in kidney, heart and liver, respectively.Conclusions:These data provide significant baseline information aboutToxoplasmapathogenesis, vaccine monitoring and drug efficiency.
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