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目的建立高效液相色谱法测定益康胶囊中红景天苷含量的方法,为保健品中红景天的测定提供检测和质量控制参考。方法采用甲醇对样品进行超声处理,经色谱柱(C18,4.6 mm×250 mm,5.0μm,安捷伦),流动相:乙腈:水=9:91(V:V)等度洗脱,柱温:40℃,检测波长:215 nm,流速:1.0 m L/min,采用外标法定量。结果结果表明,红景天苷在0.016~0.16 mg/m L范围内具有良好的线性关系(r2=1.0000),检出限为0.63μg/g,定量限为2.1μg/g,精密度(RSD)为0.5%,平均回收率97.4%(n=9)。结论此法操作简单、准确、快速,可作为益康胶囊中红景天苷含量的检测方法。
OBJECTIVE To establish a HPLC method for the determination of salidroside in Yikang capsule and to provide a reference for the detection and quality control of Rhodiola. Methods Samples were sonicated with methanol and eluted with isocratic elution of acetonitrile (C18, 4.6 mm × 250 mm, 5.0 μm, Agilent) and acetonitrile: water = 9:91 (V: V) 40 ℃, detection wavelength: 215 nm, flow rate: 1.0 m L / min, using external standard method. The results showed that salidroside had a good linearity (r2 = 1.0000) in the range of 0.016-0.16 mg / m L with a detection limit of 0.63 μg / g, a limit of quantification of 2.1 μg / g, and precision (RSD ) Was 0.5% and the average recovery was 97.4% (n = 9). Conclusion This method is simple, accurate and rapid, which can be used as the detection method of salidroside in Yikang capsule.