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ＦＣＣＰ作为质子传递型氧化磷酸化解偶联剂，是一种强力的ＡＴＰ合成抑制剂。８０μｍｏｌ／Ｌ的ＦＣＣＰ可在数分钟内使细胞内的ＡＴＰ耗竭。半胱氨酸可使ＦＣＣＰ引起的ＡＴＰ，氧消耗量和乳酸脱氢酶逸出率等改变恢复正常。半胱氨酸对ＦＣＣＰ的解毒作用是特异的和与剂量有关，而其他含巯基剂如：还原型谷胱甘肽和二硫苏糖醇（ＤＴＴ）则不能干扰ＦＣＣＰ对细胞线粒体功能的影响。
FCCP is a potent inhibitor of ATP synthesis as a proton-transphosphorylating uncoupler. FCCP at 80 μmol / L depletes intracellular ATP in minutes. Cysteine can FCCP-induced changes in ATP, oxygen consumption and lactate dehydrogenase return to normal. Cysteine detoxification of FCCP is specific and dose dependent, whereas other thiol-containing agents such as reduced glutathione and dithiothreitol (DTT) do not interfere with the effects of FCCP on cellular mitochondrial function.