为了研究谷蛋白胚乳特异性表达启动子在我国栽培稻品种中的表达模式 ,将UidA基因分别置于水稻谷蛋白GluA 2基因 750bp和 2 3kb上游序列下游 ,利用农杆菌转化法导入栽培稻品种中花 8号并获得转基因植株。Southernblot检测表明 ,UidA基因已经整合到水稻基因组当中并以单拷贝存在。Northernblot检测表明 ,开花后 13～15d和 11～ 13d ,UidA基因和水稻内源的GluA 2基因的表达量分别达到最高 ,随后逐渐降低。对转基因植株种子的GUS染色表明 ,UidA基因仅在胚乳中表达 ,在糊粉层中GUS表达量最高。测定了 2 3kb和 750bp转基因植株种子的GUS活性 ,结果表明前者的GUS活性是后者的 2～ 3倍。序列分析表明 ,位于GluA 2基因转录启始位点上游 2170bp的G box可能是一个与表达量相关的顺式调控元件 In order to study the expression pattern of gluten endosperm specific promoters in Chinese cultivated rice varieties, the UidA gene was placed in the downstream of 750bp and 23kb upstream of GluA 2 gene of rice, and introduced into cultivated rice varieties by Agrobacterium tumefaciens transformation Flower 8 and get transgenic plants. Southern blot analysis showed that the UidA gene has been integrated into the rice genome and exists as a single copy. The results of Northern blot showed that the expression levels of UidA gene and rice endogenous GluA 2 gene reached the highest at 13 ~ 15d and 11 ~ 13d after flowering respectively and then decreased gradually. GUS staining of transgenic plants showed that the UidA gene was expressed only in the endosperm and highest in the aleurone layer. The GUS activities of seeds of 2 3kb and 750bp transgenic plants were determined. The results showed that the GUS activity of the former was 2-3 times higher than that of the latter. Sequence analysis showed that the G box located at 2170 bp upstream of GluA 2 gene transcription initiation site may be a cis-regulatory element related to the expression level