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农产品细胞内的ATP含量水平直接反映细胞的活性,在产后贮藏过程中可作为农产品新鲜度和品质的一种评价指标。采用紫外-可见-近红外分光光度计UV-3600获取菠菜叶片样本的光谱数据,并采用常规物理化学方法和萤火虫荧光素酶生物发光技术制备和测定细胞原生质体悬浮液及其细胞原生质体的ATP含量。利用person相关分析确定关键特征波长,并建立基于298nm紫外光和730nm近红外光两个特征波长菠菜叶片光谱反射率的细胞原生质体ATP含量的预测模型。结果分析表明,298nm紫外光和730nm近红外光两个特征波长具有预测细胞原生质体ATP含量的潜力(R2=0.802 9和0.901)。提出的基于光谱技术的蔬菜细胞ATP含量检测方法为准确、快速、无损的蔬菜新鲜度评价提供一种新的技术途径。
The content of ATP in the cells of agricultural products directly reflects the activity of cells, which can be used as an indicator of the freshness and quality of agricultural products during postpartum storage. Spectroscopic data of spinach leaf samples were obtained using a UV-3600 UV-Vis-NIR spectrophotometer and ATP of the cell protoplast suspension and its cell protoplasts was prepared and assayed using conventional physico-chemical methods and firefly luciferase bioluminescence content. The correlation analysis of person was used to determine the key characteristic wavelength, and the prediction model of ATP content in cell protoplasts based on spectral reflectance of spinach leaves with two characteristic wavelengths of 298nm UV light and 730nm near-infrared light was established. The results show that the two characteristic wavelengths of 298nm UV and 730nm NIR have the potential of predicting the ATP content of cell protoplasts (R2 = 0.802 9 and 0.901). The proposed method for detecting ATP content in vegetable cells based on spectroscopy provides a new technical approach for accurate, rapid and nondestructive evaluation of freshness of vegetables.