目的检测云南恶性疟原虫多药抗性基因 (Pfmdr1 ) Asn 86Tyr的多态性 ,探索其与氯喹敏感性表现型的关系。方法用等位基因特异 PCR和限制性酶切片段长度分析技术 (AS-PCR/RFLP)。结果在 86% (1 9/2 2 )的氯喹抗性分离株中检测到 Pfmdr1基因的 Asn86Tyr两种多态性 ,携带编码 86Tyr多态变异的占 50 % ,而含编码 Asn86多态的也是 50 %。结论在云南氯喹抗性高度流行区 ,Pfmdr1基因 Asn86Tyr的多态性是普遍存在的 Objective To detect the polymorphism of Pfmdr1 Asn 86Tyr gene in Yunnan and to explore its relationship with chloroquine sensitive phenotype. Methods Using allele-specific PCR and restriction fragment length analysis (AS-PCR / RFLP). Results Two Asn86Tyr polymorphisms of the Pfmdr1 gene were detected in 86% (1 of 9/2 2) chloroquine resistant isolates, carrying 50% of the 86Tyr polymorphic variants and also 50 %. Conclusion The polymorphism of Asn86Tyr in Pfmdr1 gene is highly prevalent in the highly endemic areas of chloroquine resistance in Yunnan