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目的探讨汽车尾气染毒动物模型的构建方法,并初步研究其对雄性C57BL/6小鼠生殖系统的影响。方法设计构建汽车尾气染毒装置,观测该装置内氧气含量的变化和小鼠生存状态,明确通入汽车尾气的时间和间隔;为了评估本染毒装置的可靠性,将24只C57BL/6小鼠随机分成染毒组和对照组,染毒组予以汽车尾气暴露,而对照组予以正常空气。40 d后对比2组生殖腺质量的变化、精子活力、精子数目和精子畸形率,并检测血和睾丸中活性氧含量(ROS)。结果向染毒装置持续4 min通入汽车尾气,此时装置内氧气含量为(17.33±0.09)%,间隔20 min后装置内氧气的含量接近空气中的含量。染毒模型构建结束后,染毒组小鼠的生殖腺质量、精液质量明显下降,而血和睾丸ROS的含量均高于对照组。结论该研究为构建汽车尾气的染毒模型提供了方法且效果良好,明确了汽车尾气染毒后可损伤生殖系统。
OBJECTIVE: To investigate the method of establishing animal model of car tail gas exposure and to study its effect on the reproductive system of male C57BL / 6 mice. Methods To design a vehicle exhaust poisoning device, observe the change of oxygen content in the device and the survival status of mice, and determine the time and interval of vehicle exhaust. In order to evaluate the reliability of the device, 24 C57BL / 6 mice The rats were randomly divided into exposure group and control group. The exposure group was exposed to vehicle exhaust while the control group was given normal air. After 40 days, the changes of gonadal quality, sperm motility, number of sperm and sperm deformity were compared between the two groups, and the reactive oxygen species (ROS) in blood and testis were detected. Results The vehicle exhaust gas was introduced into the device for 4 min. The oxygen content in the device was (17.33 ± 0.09)%. After 20 min, the content of oxygen in the device was close to the air content. After the model was established, the gonadal quality and sperm quality of the exposed mice decreased significantly, while the blood and testicular ROS levels were higher than the control group. Conclusion This study provides a method and a good effect for building a model of vehicle tail gas exposure. It is clear that the vehicle tail gas can damage the reproductive system after being exposed to the tail gas.