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结扎大鼠冠状动脉前降支,制造早期(5 min～24h)心肌梗塞模型。用肌红蛋白的免疫组化法(PAP法)检测大鼠心肌梗塞区肌红蛋白脱失(缺染)情况,结果:冠状动脉结扎后30min,梗塞区心肌开始出现不完全性缺染;结扎2～6h,梗塞区心肌缺染范围扩大,呈带状、条状、片状,多数为不完全性缺染;结扎10～24h,梗塞区心肌缺染范围达全层。多数属完全性缺染,与非梗塞区心肌分界清晰。结论:肌红蛋白PAP法对早期心肌梗塞的病理学诊断具有应用价值。
The left anterior descending coronary artery was ligated to produce an early (5 min to 24 h) myocardial infarction model. Myoglobin immunohistochemistry (PAP method) was used to detect myoglobin depletion in the myocardial infarct region. Results: 30 minutes after coronary artery ligation, infarct myocardium began to appear incompletely stained; 2 to 6 h, the myocardial insufficiency of the infarcted area was expanded, showing strips, strips, and lamellae. Most of them were incomplete insufficiency; ligation was performed for 10 to 24 hours. The myocardial insufficiency in the infarcted area reached the full thickness. Most of the genus were completely hypochromic, and the myocardium was clearly defined in the non-infarcted area. Conclusion: The myoglobin PAP method has application value in the pathological diagnosis of early myocardial infarction.