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本文报道酶重活化剂环己磷定(HGG-42-I)水溶液稳定性测定法——紫外分光光度法。对该药在水溶液中的水解机理进行了初步探讨,认为水解是由于分子中肟基团的分解所引起的。测定了该药各pH值水溶液的紫外吸收光谱和该药酸性、碱性水解产物的紫外吸收光谱,观察到在碱性水溶液中于352nm测定HGG-42-I含量,其酸性和碱性水解产物均不干扰测定。比较了该药在各pH值碱性水溶液中的稳定性,确定测定该药含量的最佳pH值应在10以下。对加有50%分解产物的HGG-42-I水溶液的测定,平均回收率为99.74%,标准偏差为±0.13%,变异系数为±0.13%。
This article reports the determination of the stability of the enzyme heavy activator cyclohexyl phosphoridine (HGG-42-I) aqueous solution - UV spectrophotometry. The mechanism of hydrolysis of this drug in aqueous solution was preliminarily discussed. It is believed that the hydrolysis is caused by the decomposition of the oxime group in the molecule. The UV absorption spectrum of the aqueous solution of each pH value of the drug and the UV absorption spectrum of the acidic and alkaline hydrolyzate of the drug were measured. The content of HGG-42-I in the aqueous alkaline solution was observed, and the acidic and basic hydrolyzate Do not interfere with the determination. The stability of the drug in alkaline aqueous solution was compared, and the optimum pH for determination of the drug content should be below 10. The average recovery of HGG-42-I aqueous solution with 50% decomposition product was 99.74%, the standard deviation was ± 0.13% and the coefficient of variation was ± 0.13%.