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目的:探讨紫草素影响子宫内膜异位症(EMs)小鼠模型RANTES募集单核细胞、抑制异位内膜生长的作用机制。方法:建立人鼠嵌合型EMs动物模型,设大(10mg/kg)、中(5mg/kg)、小(2.5mg/kg)剂量紫草素治疗组,治疗28d。另设PBS阴性对照组、达菲林阳性对照组,采用形态学方法比较紫草素对EMs小鼠模型移植人子宫蜕膜生长抑制的情况;体内趋化实验评价紫草素对RANTES募集单核细胞的影响。结果:不同剂量紫草素及达菲林均能抑制小鼠体内人子宫蜕膜的生长,与PBS组比较,差异显著(P<0.05)。其中大、中剂量组异位灶缩小最显著(分别为P=0.000和P=0.001),其次为达菲林组(P=0.003)和小剂量组(P=0.011),各组间比较差异无显著意义(P>0.05)。紫草素明显抑制rhRANTES对U937细胞的趋化作用(P<0.05)。结论:人鼠嵌合型EMs动物模型可用于EMs的研究。紫草素能抑制异位灶生长,该作用可能通过抑制趋化因子募集单核细胞至腹腔,减轻腹腔炎症而起作用。
Objective: To investigate the effect of shikonin on the recruitment of monocytes and the inhibition of ectopic endometrial growth by RANTES in a mouse model of endometriosis (EMs). METHODS: An animal model of human and mouse chimeric EMs was established. The large (10 mg/kg), middle (5 mg/kg), and small (2.5 mg/kg) doses of shikonin treatment group were treated for 28 days. A PBS-negative control group and a daphneline-positive control group were set up. Morphological methods were used to compare the inhibitory effect of shikonin on uterine decidual growth in EMs mouse models. In vivo chemotaxis assay evaluated shikonin for RANTES recruitment of monocytes. Impact. RESULTS: Different doses of shikonin and dafilin inhibited the growth of human uterine decidua in mice, which was significantly different from that of PBS group (P<0.05). The ectopic focus in the large and middle dose groups was the most significant (P = 0.000 and P = 0.001, respectively), followed by the Dafflin group (P = 0.003) and the low dose group (P = 0.011). There was no difference between the groups. Significant (P>0.05). Shikonin significantly inhibited the chemotactic effects of rhRANTES on U937 cells (P<0.05). Conclusion: The human and mouse chimeric EMs animal model can be used for the study of EMs. Shikonin can inhibit the growth of ectopic foci, which may act by inhibiting the recruitment of chemokines to mononuclear cells to the abdominal cavity and reducing inflammation in the abdominal cavity.