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利用60Coγ射线以不同剂量照射硬粒小麦-簇毛麦双二倍体即将成熟的花粉,将其授于母本中国春,创造出一批包含小麦-簇毛麦易位染色体的材料,对这些材料用中国春进行连续回交或自交,可有效保留簇毛麦染色体片段,实现外源基因的转移。研究结果表明,60Coγ射线照射花粉后产生易位染色体的频率因剂量不同而有显著差异,12 Gy和8 Gy剂量照射后杂交的M1群体中,产生小麦-簇毛麦易位染色体的单株分别占调查总数的76.7%和50.0%,均显著高于用其他方法创造易位的频率,并且12 Gy较8 Gy产生了更优的易位类型;创制的易位染色体有67.6%可以从M1传递到BC1,BC1的易位染色体有96.4%可传递到BC2;在回交后代中,加以人为选择,整条簇毛麦染色体很快丢失,至BC2F2即有纯合易位株出现。
Using 60Coγ-rays to irradiate the maturing pollen of durum wheat-tussilahum at different doses, we give it to Chinese Spring of the female parent to create a batch of materials containing chromosomes of wheat-tufted wheat translocation, The materials were continuously backcrossed or selfed with Chinese Spring, which can effectively retain the chromosome segments of the cluster mahogany and achieve the transfer of foreign genes. The results showed that the frequencies of translocation chromosomes produced by 60Coγ-ray irradiation were significantly different due to the different dosage. In the M1 population that hybridized with 12 Gy and 8 Gy doses, the individuals producing wheat-cluster-wool translocation chromosomes were different Accounting for 76.7% and 50.0% of the total number of surveys, were significantly higher than other methods to create the frequency of translocations, and 12 Gy produced better translocation than 8 Gy type; 67.6% of translocation chromosome created transfer from M1 BC1, 96.4% of BC1 translocated chromosomes can be transferred to BC2; in the backcross progenies, to be artificially selected, the entire cluster of hairy maize chromosomes quickly lost to BC2F2 that there homozygous translocations.