通过体外矿化条件观察培养兔骨髓细胞的成骨能力，结果显示：①第３代骨髓细胞碱性磷酸酶染色，７％～８％阳性；②倒置显微镜下观察，细胞传代５～６ｄ后，长满培养瓶的底部，约１０ｄ开始叠层生长，为局灶状，中央出现较多颗粒样改变，１４～１５ｄ时，培养瓶底部肉眼可见白色小结节形成，并逐渐增大；③细胞连续培养３０ｄ，Ｖｏｎ－Ｋａｏｓａ染色显示有局灶状钙盐沉积；④ＳＥＭ观察，细胞呈叠层生长，形成粗大的胶原纤维束，上有钙盐沉淀，３０ｄ时，形成骨小梁样结构。上述结果表明，骨髓细胞体外成骨可以用于组织工程 The osteogenic ability of cultured rabbit bone marrow cells was observed by in vitro mineralization conditions. The results showed that: (1) The third generation of bone marrow cells were stained with alkaline phosphatase (7% -8%); (2) The cells were observed under an inverted microscope 5 to 6 days after passage, Covered with the bottom of the flask, about 10d began to grow in layers, as the focal shape, the central appear more granular change, 14 ~ 15d, the bottom of the culture flask visible white nodules visible and gradually increased; ③ cells After 30 days of continuous culture, Von-Kaosa staining showed focal calcium deposition. (4) SEM observation showed that the cells grew in lamella and formed coarse collagen fiber bundles with calcium salts precipitated. At 30 days, trabecular-like structures were formed. The above results show that bone marrow cells in vitro osteogenesis can be used for tissue engineering