论文部分内容阅读
[目的]采用母婴分离联合束缚应激法建立腹泻型肠易激综合征大鼠模型,并对其进行初步评价。[方法]刚出生1d的SD幼鼠随机分为4组:对照组(NH),母婴分离组(NMS),束缚应激组(RS),母婴分离联合束缚应激组(NMS+RS)。造模结束后,分别观察其体重变化、排便情况及腹壁回撤反射(AWR)。[结果]模型组大鼠体重均较NH组低(P<0.01),且二因素组(NMS+RS组)较单因素组(NMS组,RS组)更低,差异均有统计学意义(P<0.05)。大鼠粪便Bristol分级评分及粪便含水量,模型组均较NH组高,结果有统计学意义(P<0.05);二因素组较单因素组高,差异有统计学意义(P<0.01)。在10mmHg及20mmHg时,4组之间腹壁回撤反射(AWR)评分结果近似,差异无统计学意义(P>0.05);40~80mmHg时,NMS组,NMS+RS组AWR评分均较NH组高,差异有统计学意义(P<0.05);60mmHg及80mmHg时,NMS+RS组AWR评分较RS组高,二者结果差异有统计学意义(P<0.05)。[结论]母婴分离法、束缚应激法均能在一定程度上模拟腹泻型肠易激综合征的临床症状及(或)内脏高敏感性,采用二者相结合(母婴分离联合束缚应激)的方法建立的大鼠模型与任何单一方法比较,更加符合腹泻型肠易激综合征的症状及内脏高敏感性的特征。
[Objective] To establish a rat model of diarrhea-predominant irritable bowel syndrome (IBS) using maternal-fetal separation combined with restraint stress method and to evaluate it. [Methods] SD young rats were randomly divided into 4 groups: control group (NH), maternal separation group (NMS), restraint stress group (RS), maternal and infant joint restraint stress group ). After modeling, the body weight, defecation and abdominal wall reflex (AWR) were observed. [Results] Compared with NH group, the body weight of model group was lower than that of NH group (P <0.01), and the level of NMS + RS group was lower than that of single factor group (NMS group and RS group) P <0.05). The stool Bristol grading score and stool water content in model group were higher than those in NH group (P <0.05). The two factor groups were higher than the single factor group (P <0.01). At 10mmHg and 20mmHg, the AWR scores of the four groups were similar (P> 0.05), while the AWR scores of the NMS group and the NMS + RS group were significantly higher than those of the NH group at 40 ~ 80mmHg (P <0.05). At 60mmHg and 80mmHg, the AWR score of NMS + RS group was higher than that of RS group (P <0.05). [Conclusion] Both mother-infant separation method and restraint stress method can simulate the clinical symptoms and / or visceral hypersensitivity of diarrhea-predominant irritable bowel syndrome to a certain extent by combining the two Stimulated) approach to establish a rat model compared with any single method, more in line with diarrhea-predominant irritable bowel syndrome and visceral hypersensitivity characteristics.