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目的 将复杂的人精子体外穿卵试验进行改进和完善 ,使之利于常规开展。方法 将金黄地鼠行超排卵 ,并将取得的金黄地鼠卵子进行去透明带处理 ,与体外处理并获能的人精子放在一起受精 ,通过低渗、固定染色制片 ,观察人精子穿透率。结果 用该法检测了 10例有正常生育能力精子穿透率 ,范围在 30 %～ 80 % ;2 0例精液常规正常原发不育者精子穿透率在 10 %以下。结论 改进制片后穿卵试验是用于检测人精子受精能力一种方法。
Objective To improve and perfect the in vitro test of human in vitro sperm penetration so that it is conducive to the routine development. Methods The golden hamster was ovariectomized and the golden hamster ovum obtained was treated with zona pellucida. The spermatozoa were treated with in vitro fertilization and fertilized. The spermatozoa were observed by hypotonic and fixed staining Transparency. Results The method was used to detect the sperm penetration rate of 10 normal fertility spermatozoa in the range of 30% -80%. The sperm penetration rate of 20 normal spermatozoa were less than 10%. Conclusion Improving the ovulation test after filming is a method for detecting human sperm fertilization ability.