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目的探讨宫颈病变中中心体异常扩增与高危人乳头状瘤病毒(high-risk human papillomavirus,HR-HPV)感染的关系。方法对46例宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)(CIN组)、43例宫颈鳞状细胞癌患者(宫颈鳞癌组)及20例正常宫颈组织者(对照组)进行宫颈脱落细胞的HR-HPV基因型检测,另采用间接免疫荧光染色技术检测中心体。结果对照组、CIN组及宫颈鳞癌组HR-HPV感染阳性率依次增高,分别为5.00%、78.26%、93.02%,组间比较差异均有统计学意义(P<0.05);CIN组与宫颈鳞癌组细胞中心体异常细胞率分别为(1.76±1.74)%、(4.46±2.08)%,2组比较差异有统计学意义(P<0.05);双变量相关分析显示宫颈病变中,中心体异常细胞率和HR-HPV感染呈正相关(r=0.452,P=0.000)。结论宫颈鳞癌发生、发展过程中,中心体异常扩增与HR-HPV感染密切相关;HR-HPV感染可能通过引起中心体的异常扩增诱发宫颈癌变。
Objective To investigate the relationship between abnormal amplification of centrosome in cervical lesions and high-risk human papillomavirus (HR-HPV) infection. Methods Cervical exfoliated cells were isolated from 46 cases of cervical intraepithelial neoplasia (CIN), 43 cases of cervical squamous cell carcinoma (SCC) and 20 cases of normal cervical tissue (control group) HR-HPV genotype detection, the other using indirect immunofluorescence staining of centrosomes. Results The positive rates of HR-HPV infection in control group, CIN group and cervical squamous cell carcinoma group were successively higher (5.00%, 78.26%, 93.02% respectively), with significant difference between the two groups (P <0.05) The rate of abnormal cell in centromere of squamous cell carcinoma was (1.76 ± 1.74)% and (4.46 ± 2.08)%, respectively. The difference between the two groups was statistically significant (P <0.05). Bivariate correlation analysis showed that in cervical lesions, centrosome Abnormal cell rate was positively correlated with HR-HPV infection (r = 0.452, P = 0.000). Conclusions In the development of cervical squamous cell carcinoma, abnormal amplification of central body is closely related to HR-HPV infection. HR-HPV infection may induce cervical carcinogenesis by causing abnormal amplification of centrosome.