论文部分内容阅读
超音速火焰(HVOF)喷涂纳米氧化钛加10%羟基磷灰石(HA)(n-TiO2-10%HA)粉末制备的生物医学涂层有望取代大气等离子喷涂的HA涂层。选用这种方法的原因为TiO2在人体内的稳定性很高(即没有溶解),另外,在Ti-6Al-4V的基板上的结合强度超过APS-HA涂层2倍。为了探索这些新材料和涂层的生物表现,在超音速火焰喷涂的n-TiO2和n-TiO2-10%HA的涂层表面上进行了从1到21天的人体间质干细胞(hMSCs)培养,APS的HA涂层和未涂层的Ti-6Al-4V合金基体作为对照。对hMSCs的活性特征进行了以下分析:阿拉莫尔艾迪布尔细胞的繁殖;生化碱性磷酸酶(ALP)活性的分析;细胞骨架组织(通过荧光/共聚焦显微镜)及细胞/基体的互动(通过扫描电子显微镜(SEM))。细胞繁殖和生化分析表明,在n-TiO2-10%HA涂层上培养的hMSCs表现出了类似的或优于APS-HA涂层上的hMSCs生物活性。细胞骨架组织在超音速火焰喷涂n-TiO2-10%HA涂层表面上具有更高的细胞繁殖和附着程度。这些结果对下一代高性能长寿命热喷涂生物涂层具有重要意义。
Biomedical coatings prepared by HVOF spraying of nano-titanium oxide plus 10% hydroxyapatite (HA) (n-TiO2-10% HA) powder are expected to replace the atmospheric plasma sprayed HA coating. The reason for choosing this method is the high stability (ie no dissolution) of TiO2 in the human body. In addition, the binding strength on the Ti-6Al-4V substrate is more than twice that of the APS-HA coating. To explore the biological performance of these new materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on supersonic flame sprayed n-TiO2 and n-TiO2-10% HA coated surfaces , APS HA coating and uncoated Ti-6Al-4V alloy matrix as a control. Activity characteristics of hMSCs were analyzed as follows: Propagation of Alabama Eddier cells; analysis of biochemical alkaline phosphatase (ALP) activity; interaction of cytoskeletal tissue (via fluorescence / confocal microscopy) and cell / matrix By scanning electron microscopy (SEM)). Cell proliferation and biochemical analysis showed that hMSCs cultured on n-TiO2-10% HA coating exhibited similar or superior biological activity to hMSCs on APS-HA coating. Cytoskeleton tissue has a higher degree of cell multiplication and attachment on the surface of the supersonic flame spray n-TiO2-10% HA coating. These results are of great importance to the next generation of high performance long life thermal sprayed biological coatings.