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采用氯胺Ｔ法对人降钙素（ｈＣＴ，以下简称ＣＴ）进行１２５Ｉ标记，用ＳｅｐｈａｄｅｘＧ－５０柱分离纯化，得到比活度＞９．５ＴＢｑ／ｇ、放化纯度＞９５％、与过量抗体结合率为（８５—９１）％的１２５Ｉ－ＣＴ。建立了稳定的冻干系列标准及简便、快速的分析方法。标准曲线范围为８．８—３５１ｐｍｏｌ／Ｌ，在该范围内测定人血清的平均回收率为（９５．５—１０２．０）％，高浓度血清的稀释相关系数为ｒ＝０．９９９３—０．９９９８，灵敏度为５．３ｐｍｏｌ／Ｌ，批内变异系数为（３．２—８．６）％，批间变异系数为（４．７—１０．４）％，非特异结合率为（２．４—４．７）％，健康人血清ＣＴ含量的正常值为１１．７±６．４ｐｍｏｌ／Ｌ。
The chloramine T method was used to label human calcitonin (hCT, hereinafter referred to as CT) for 125I and purified by SephadexG-50 column. The specific activity> 9.5TBq / g, radiochemical purity> 95% 125I-CT with a binding rate of (85-91)%. Established a series of stable freeze-drying standards and simple and rapid analysis. The standard curve ranged from 8.8 to 351 pmol / L. The average recovery of human serum in this range was (95.5-102.0)% and the dilution of high serum was r = 0.9993-0 .9998, the sensitivity was 5.3 pmol / L, the intra-assay CV was 3.2-8.6%, the inter-assay CV was 4.7-10.4%, and the non-specific binding rate was 2 .4-4.7)%, normal healthy serum CT content of 11.7 ± 6.4pmol / L.