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目的探讨沙尘暴颗粒物(PM10、PM2.5)对人肺成纤维细胞的细胞毒性。方法分别用50、100、150、200μg/ml的PM10和PM2.5对人肺成纤维细胞进行染毒,24h后测定细胞线粒体琥珀酸脱氢酶活力、细胞外乳酸脱氢酶和酸性磷酸酶的漏出量。结果50μg/ml以上浓度的沙尘暴颗粒物可剂量依赖性地抑制人肺成纤维细胞的线粒体琥珀酸脱氢酶活力。在更高染毒浓度,沙尘暴颗粒物可引起细胞外乳酸脱氢酶和酸性磷酸酶的漏出增加。结论沙尘暴颗粒物对人肺成纤维细胞可产生明显的毒性作用。线粒体可能是沙尘暴颗粒物毒作用的敏感部位。
Objective To investigate the cytotoxicity of dust particles (PM10, PM2.5) on human lung fibroblasts. Methods Human lung fibroblasts were exposed to PM10 and PM2.5 at doses of 50, 100, 150 and 200 μg / ml, respectively. After 24 hours, mitochondrial succinate dehydrogenase activity, extracellular lactate dehydrogenase and acid phosphatase Of leakage. Results Sand dust particles above 50 μg / ml could dose-dependently inhibit mitochondrial succinate dehydrogenase activity in human lung fibroblasts. At higher exposure levels, dust particles can cause an increase in leakage of extracellular lactate dehydrogenase and acid phosphatase. Conclusion Sand dust particles can produce obvious toxic effects on human lung fibroblasts. Mitochondria may be the sensitive part of the dust particles of dust storms.