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目的建立同时测定侧柏叶中杨梅苷、槲皮苷、穗花杉双黄酮和扁柏双黄酮含量的方法,为侧柏叶的质量控制和开发利用提供依据。方法采用HPLC法。色谱柱为Waters ODS C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-体积分数为0.5%乙酸溶液,梯度洗脱,流速为0.8 mL·min-1,检测波长为340 nm,柱温为室温。结果杨梅苷、槲皮苷、穗花杉双黄酮和扁柏双黄酮质量浓度分别在61.2~122.4 mg·L-1(r=0.999 8)、10.56~211.2 mg·L-1(r=0.999 8)、2.85~57.0 mg·L-1(r=0.999 9)和7.54~150.8 mg·L-1(r=0.999 8)内与峰面积呈良好的线性关系;平均加样回收率分别为97.0%(RSD=2.5%)、97.1%(RSD=2.3%)、96.8%(RSD=2.1%)和97.4%(RSD=3.4%)。结论此法操作简便、快速、灵敏、准确,样品处理简便易行,适于侧柏叶的质量控制。
OBJECTIVE To establish a method for simultaneous determination of myricetin, quercitrin, biflavone and cypress flavone in oriental arborvitae leaves, and to provide basis for the quality control and exploitation of oriental arborvitae leaf. Methods HPLC method. The column was a Waters ODS C18 column (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of a 0.5% acetic acid solution and a gradient elution at a flow rate of 0.8 mL · min-1. The detection wavelength was 340 nm. The temperature is room temperature. Results The results showed that the concentrations of myricitrin, quercetin, biflavone and cypressin were 61.2-122.4 mg · L-1 (r = 0.999 8), 10.56-211.2 mg · L-1 (r = 0.999 8) The linear range was 2.85 ~ 57.0 mg · L-1 (r = 0.999 9) and 7.54-150.8 mg · L-1 (r = 0.999 8). The average recoveries were 97.0% (RSD = 2.5%), 97.1% (RSD = 2.3%), 96.8% (RSD = 2.1%) and 97.4% (RSD = 3.4%). Conclusion This method is simple, rapid, sensitive and accurate. The method of sample processing is simple and convenient, and is suitable for the quality control of oriental arborvitae.