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研究了高频率诱导荞麦愈伤组织和再生植株的组培技术。在附加 0 .5 mg/L 2 .4-D和 KT的 MS4培养基上 ,胚轴和子叶外植体愈伤组织的诱导率高达 81.3 %～ 91.2 % ,胚轴不定芽的诱导率为 3 4 .5 %～ 41.2 %。将胚轴在附加 0 .5 mg/L 2 .4—D的 MS2上预培养 15 d左右再转到 MS4上 ,不定芽诱导率可达 5 3 .3 %。不定芽也可从培养于 MS5 (附加 0 .5 mg/L KT)中的外植体上直接产生。当不定芽转到附加 0 .5 mg/L NAA的 MS7培养基上可诱导生根。本研究为进一步开展荞麦细胞工程和基因工程研究提供了较成熟的组培技术
The technique of tissue culture of callus and regenerated plants induced by high frequency was studied. On the MS4 medium supplemented with 0 .5 mg / L 2 .4-D and KT, the induction rate of callus on hypocotyl and cotyledon explants was as high as 81.3% -91.2% and the induction rate of adventitious buds on hypocotyls was 3 4 .5% ~ 41.2%. The hypocotyls were preincubated for about 15 days on MS2 supplemented with 0.5 mg / L 2 .4-D and then transferred to MS4. The induction rate of adventitious buds was 53.3%. Adventitious shoots can also be generated directly from explants cultured in MS5 (plus 0.5 mg / L KT). When adventitious buds were transferred to MS7 medium supplemented with 0.5 mg / L NAA, rooting could be induced. This study provides more mature tissue culture techniques for the further research of buckwheat cell engineering and genetic engineering