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目的研究苦参碱(MT)致神经胶质瘤U87凋亡的作用及其机制。方法培养U87细胞,通过CCK-8细胞增殖实验检测MT对U87细胞增殖的影响并确定最佳用药浓度。实验分为正常对照组(Control)和加药组(MT),利用Western blot检测2组U87细胞中热休克蛋白60(HSP60)和Caspase-3的表达水平及应用免疫荧光染色技术检测MT对U87细胞凋亡的影响。结果 0.4-0.8μg/μl的MT可以减少U87细胞的增殖,与对照组相比,0.4μg/μl的MT促进了HSP60和Caspase-3的表达,U87细胞数目明显减少(P<0.05)。结论 MT可能通过提高HSP60的表达促进U87细胞的凋亡。
Objective To investigate the role of matrine (MT) in the apoptosis of glioma U87 and its mechanism. Methods U87 cells were cultured. The proliferation of U87 cells was detected by CCK-8 cell proliferation assay and the optimal drug concentration was determined. The experiment was divided into normal control group and dosing group (MT). Western blot was used to detect the expression of heat shock protein 60 (HSP60) and caspase-3 in two groups of U87 cells and immunofluorescence staining was used to detect the effect of MT on U87 Effect of apoptosis. Results MT 0.4-0.8μg / μl could reduce the proliferation of U87 cells. Compared with the control group, 0.4μg / μl MT promoted the expression of HSP60 and Caspase-3, and significantly reduced the number of U87 cells (P <0.05). Conclusion MT may promote the apoptosis of U87 cells by increasing the expression of HSP60.