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目的:从红细胞造血生长因子的角度探讨运动性贫血的发生机理。方法:以30只雄性Wistar大鼠为实验对象,进行为期11周的实验控制,通过对大鼠实施递增负荷跑台运动建立运动性贫血模型。大鼠随机分为3组:对照组(10只)、递增负荷跑台运动组(运动组,10只)、递增负荷跑台运动+营养补充组(运动+营养组,第5周结束时即大鼠运动性贫血模型成功建立后进行营养补充,10只),应用大鼠专用ELISA试剂盒测试三组大鼠血清造血生长因子白细胞介素3(IL-3)、粒细胞集落刺激因子(G-CSF)、粒-单核细胞集落刺激因子(GM-CSF)的含量。结果:运动组大鼠血清G-CSF水平显著高于对照组(P<0.05),运动+营养组与对照组相比无显著性差异(P>0.05),而GM-CSF、IL-3含量则无明显组间差异。结果提示,运动性贫血的发生可能与G-CSF的功能相关,而营养补充对大鼠血清三种造血生长因子影响不明显。
Objective: To explore the mechanism of exercise-induced anemia from the perspective of erythrocyte hematopoietic growth factor. Methods: Thirty male Wistar rats were used for experimental control for 11 weeks. The model of exercise-induced anemia was established by increasing the load and running treadmill exercise in rats. The rats were randomly divided into 3 groups: control group (n = 10), incremental load treadmill exercise group (exercise group, n = 10), incremental load treadmill exercise + nutrition supplement group Rat model of exercise-induced anemia was established after nutritional supplementation, 10 rats). Serum levels of hematopoietic growth factors interleukin 3 (IL-3), granulocyte colony stimulating factor (G -CSF), granulocyte monocyte colony stimulating factor (GM-CSF) content. Results: The level of serum G-CSF in exercise group was significantly higher than that in control group (P <0.05), and there was no significant difference between exercise + nutrition group and control group (P> 0.05) No significant difference between groups. The results suggest that the occurrence of exercise-induced anemia may be related to the function of G-CSF, while nutritional supplementation has no obvious effect on the three hematopoietic growth factors in serum of rats.