建立了茶叶中儿茶素、表儿茶素、表没食子儿茶素和表儿茶素没食子酸酯含量的超高压液相色谱分析方法。利用75%的乙醇溶液超声提取茶叶后,选用ACQUITY UPLCTM BEHC C18柱,以乙腈与水之比为20∶80为流动相,通过二极管阵列检测器在280 nm波长处检测。该方法在质量浓度在1~100 mg/L范围内线性良好,相关系数为0.999 55~0.999 82,检出限(S/N=3)为0.485~1.75 mg/kg,相对标准偏差(RSD)为0.45%~1.3%,回收率为95.83%~108.33%。 A method for the determination of catechins, epicatechin, epigallocatechin and epicatechin gallate in tea by ultra high pressure liquid chromatography was established. After the tea leaves were extracted by ultrasonic with 75% ethanol solution, ACQUITY UPLCTM BEHC C18 column was used. The mobile phase consisted of acetonitrile and water 20:80 and detected by diode array detector at 280 nm. The method showed a good linearity in the range of 1 ~ 100 mg / L with a correlation coefficient of 0.999 55 ~ 0.999 82 and a detection limit (S / N = 3) of 0.485 ~ 1.75 mg / kg with relative standard deviation 0.45% ~ 1.3%, the recovery rate was 95.83% ~ 108.33%.