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目的研究针毛蕨(Macrothelypteris oligophlebia)化学成分及其体内外抗肿瘤活性。方法采用各种色谱技术进行分离纯化,根据理化性质和波谱数据进行结构鉴定,以MTT法测试各化合物对肿瘤细胞的体外抑制作用,并观察针毛蕨提取物对S180肉瘤小鼠肿瘤生长的影响。结果分离得到7个黄酮类化合物,分别鉴定为:荚果蕨素(1)、柚皮素(2)、柚皮素-5-O-β-D-吡喃葡萄糖苷(3)、原芹菜素(4)、去甲氧基荚果蕨素(5)、芹菜素(6)和原芹菜素-4′-O-β-D-葡萄糖苷(7)。结论所有化合物均首次从针毛蕨中分离得到;化合物4对肿瘤细胞增殖有显著的抑制作用,且呈现出良好的剂量依赖性;体内抗肿瘤试验显示针毛蕨提取物对S180肉瘤小鼠肿瘤的生长有明显的抑制作用。
Objective To study the chemical constituents of Macrothelypteris oligophlebia and its antitumor activity in vitro and in vivo. Methods The compounds were separated and purified by various chromatographic techniques. Their structures were elucidated on the basis of physico-chemical properties and spectral data. The inhibitory effect of each compound on tumor cells was tested by MTT assay. The effects of extracts from acanthopanax senticosus on tumor growth in S180 sarcoma mice were observed . Results Seven flavonoid compounds were isolated and identified as podophyllin (1), naringenin (2), naringenin -5-O-β-D-glucopyranoside (3) (4), demethoxy podophyllin (5), apigenin (6) and procididin -4’-O-β-D-glucoside (7). Conclusion All the compounds were isolated from A. spinosa for the first time. Compound 4 showed a significant inhibitory effect on the proliferation of tumor cells and exhibited a good dose-dependent manner. In vivo antitumor assays showed that the extract of acanthopanax senticosus on S180 sarcoma tumor The growth of a significant inhibitory effect.