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目的 :研究 p5 3和bcl 2在大肠肿瘤发生中的作用。方法 :应用免疫组化S P法分别检测大肠正常粘膜、腺瘤及腺癌中p5 3和bcl 2表达。 结果 :大肠腺癌 p5 3表达率高于腺瘤组 (P <0 0 5 ) ,p5 3表达与大肠癌的临床病理因素无关 (P >0 0 5 ) ,bcl 2在正常粘膜基底部上皮细胞表达 ,在腺瘤 (77 5 % )和腺癌中 (5 5 % )bcl 2表达差异显著 (P <0 0 1) ,高分化腺癌bcl 2表达率(73 7% )高于差分化腺癌 (41 2 % ) ,在腺瘤和腺癌中bcl 2和p5 3呈负相关。结论 :p5 3基因改变在大肠腺瘤癌变中起重要作用 ,大肠癌的发生与bcl 2基因在大肠腺瘤和腺癌中细胞凋亡抑制有关。
Objective : To study the role of p53 and bcl 2 in colorectal tumorigenesis. METHODS: The expression of p53 and bcl-2 in normal mucosa, adenoma and adenocarcinoma were detected by immunohistochemical SP method. Results: The expression rate of p53 in colorectal adenocarcinoma was higher than that in adenoma group (P < 0.05). The expression of p53 was not associated with the clinicopathological factors of colorectal carcinoma (P > 0.05). Bcl 2 was found in normal mucosal basal epithelial cells. The expression of bcl 2 in adenomas (77.5%) and adenocarcinomas (5.5%) was significantly different (P <0 01). The expression of bcl 2 in highly differentiated adenocarcinomas (73.7%) was higher than that of the differential glands. Cancer (41 2%) was negatively correlated with bcl 2 and p53 in adenomas and adenocarcinomas. Conclusion : The p53 gene plays an important role in the carcinogenesis of colorectal adenomas. The occurrence of colorectal cancer is associated with the inhibition of apoptosis of the bcl 2 gene in colorectal adenomas and adenocarcinomas.