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国外自80年代初期应用ABC方法于免疫组织化学的研究以来,大大地推动了细胞学、免疫学及病理学等学科的发展。我室应用ABC方法进行了一些研究工作,已标记了近万张组织切片或细胞滴片,比PAP方法敏感性高。为了解决进口试剂价格昂贵以及推广应用上的困难,我们从1985年起应用上海医科大学化学教研室自制的LAB系统(标记的卵白素-生物素-辣根过氧化酶系统)进行免疫化学标记的研究,现将结果报道如下。
Since the application of ABC method to immunohistochemistry in the early 1980s, the development of cytology, immunology, and pathology has been greatly promoted. Our laboratory applied ABC method to carry out some research work and has marked tens of thousands of tissue sections or cell fragments, which is more sensitive than PAP. In order to solve the problem of expensive imported reagents and difficulties in popularization and application, we applied the LAB system (marked avidin-biotin-horseradish peroxidase system) that was developed by the Chemistry Teaching and Research Section of Shanghai Medical University for immunochemical labeling since 1985. The results are reported below.