猪皮胶原肽的抗氧化活性研究

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目的:研究不同分子量猪皮胶原肽的抗氧化特性,筛选出抗氧化活性较强的组分。方法:采用仿生酶解技术酶解新鲜猪皮得到酶解原液,酶解原液经超滤得到M〈1 k Da、1 k Da3 k Da 3个不同分子量肽段,猪皮经水提取得到猪皮水提液,上述5组分分别冻干,得到冻干粉。对不同分子量范围的猪皮胶原肽5组分进行肽含量测定和抗氧化性研究,包括对DPPH·自由基、O2-·自由基及·OH自由基的清除实验。结果:各组分的肽含量分别为酶解原液组分64.62%、1-3 k Da组分62.63%、大于
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