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目的:研究金丝桃苷对乙醇致小鼠急性酒精性肝损伤的保护作用。方法:昆明种小鼠随机分为正常组、模型组、联苯双脂组(5.625 mg/kg)和金丝桃苷(12.5、25、50 mg/kg)剂量组。每日灌胃给药1次,连续7 d。末次给药1 h后,除正常组外,分别灌胃给予50%乙醇14 ml/kg复制酒精性肝损伤模型。复制模型12 h后,处理动物,测定小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)和γ-谷氨酰转移酶(GGT)以及甘油三酯(TG)和胆固醇(TC)水平;测定肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)和谷胱甘肽(GSH)含量。苏木素-伊红(HE)染色,观察肝组织病理学变化。结果:与正常组比较,模型组小鼠清ALT,AST,GGT及TG水平显著升高,肝组织SOD活性及GSH含量显著降低,MDA含量显著升高,病理学结果显示模型组肝组织损伤明显。与模型组比较,金丝桃苷(25、50 mg/kg)剂量组均能降低血清中ALT、AST,GGT和TG水平以及肝组织中MDA含量,同时升高SOD活性和GSH含量;金丝桃苷(12.5 mg/kg)剂量组能降低血清中ALT、AST水平,升高SOD活性;金丝桃苷各剂量组(12.5、25、50 mg/kg)均能减轻肝损伤的病变程度。结论:金丝桃苷对乙醇所致的小鼠急性酒精性肝损伤具有保护作用,其机制可能与抗氧化作用和抑制脂质过氧化损伤有关。
Objective: To study the protective effect of hyperoside on ethanol-induced acute alcoholic liver injury in mice. Methods: Kunming mice were randomly divided into normal group, model group, biphenolipid group (5.625 mg / kg) and hyperin (12.5, 25, 50 mg / kg) Gavage administered once daily for 7 days. One hour after the last administration, except for the normal group, rats were randomly divided into three groups: 50% ethanol and 14 ml / kg orally, respectively. After 12 h replication of the model, the animals were treated and serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and γ-glutamyl transferase GGT), triglyceride (TG) and cholesterol (TC) were measured. The activity of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) Hematoxylin - eosin (HE) staining, liver histopathological changes were observed. Results: Compared with the normal group, the levels of ALT, AST, GGT and TG in the model group were significantly increased, the activity of SOD and the content of GSH in the liver tissue were significantly decreased, the content of MDA was significantly increased, the pathological results showed that the model group had obvious liver damage . Compared with the model group, hyperoside (25,50 mg / kg) could decrease the level of ALT, AST, GGT and TG in serum and the content of MDA in liver tissue, and increase the activity of SOD and the content of GSH In the dose of 12.5 mg / kg, the levels of ALT and AST in serum increased and the activity of SOD increased. The dosage of 12.5, 25 and 50 mg / kg of oxaliplatin could reduce the severity of liver injury. CONCLUSION: Hyperin has a protective effect on alcohol-induced acute alcoholic liver injury in mice. The mechanism may be related to its antioxidation and inhibition of lipid peroxidation.