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目的:研究蕨麻正丁醇部位对缺氧损伤心肌细胞凋亡及Caspase 3、Caspase 9表达的抑制作用。方法:建立大鼠原代培养心肌细胞缺氧模型,HE染色观察细胞组织形态,PI-Hochesst3342染色观察细胞凋亡,RT-PCR技术及免疫细胞化学染色方法检测细胞Caspase 3、Caspase 9 mRNA及其产物蛋白水平。结果:与模型组比较,正丁醇部位各剂量组Caspase 3、Caspase 9 mRAN表达水平均显著降低(P<0.05或P<0.01);高剂量组Caspase 3、Caspase 9蛋白水平显著降低(P<0.01)。结论:蕨麻正丁醇部位可能通过减弱缺氧诱导的心肌细胞Caspase级联反应,抑制心肌细胞凋亡。
OBJECTIVE: To study the effects of n-butanol fraction on the apoptosis of cardiomyocytes and the expression of Caspase 3 and Caspase 9 induced by hypoxia. Methods: Primary cultured rat cardiomyocytes hypoxia model was established. HE staining was used to observe the cell morphology. PI-Hochesst3342 staining was used to observe apoptosis. RT-PCR and immunocytochemical staining were used to detect the mRNA and protein expression of Caspase 3 and Caspase 9 Product protein levels. Results: Compared with the model group, the expressions of Caspase 3 and Caspase 9 mRAN in the n-butanol group were significantly decreased (P <0.05 or P <0.01), and the levels of Caspase 3 and Caspase 9 in the high dose group were significantly decreased (P < 0.01). CONCLUSION: The n-butanol fraction of Pteris nivalis may inhibit cardiomyocyte apoptosis by attenuating hypoxia-induced Caspase cascade in cardiomyocytes.