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本文采用Ｇ显带技术研究了辐射致大鼠气管上皮（ＲａｔＴｒａｃｈｅａｌＥｐｉｔｈｅｌｉｕｍ，ＲＴＥ）细胞体外恶性转化过程中染色体非随机异常，结果：（１）细胞受照后，在传代早期以亚二倍体细胞为主，随传代次数增加其比例逐渐降低，在传代后期以多倍体细胞为主，这种变化趋势可能与细胞转化进展有关；（２）在转化细胞，１５号染色体长臂重排（１５ｑ＋）为其特征性染色体改变，它可能在ＲＴＥ细胞恶性转化过程中起重要作用；（３）Ｍａｒｋｅｒ染色体Ｍ１、Ｍ２和Ｍ３随传代次数增加发生频率逐渐升高，特别是Ｍ１和Ｍ２在致瘤细胞高频出现（＞９０％），提示可能与细胞转化进展有关。
In this study, G-banding technique was used to study the non-random chromosomal abnormalities during the malignant transformation of Rat Tracheal Epithelium (RTE) cells induced by radiation. Results: (1) After the cells were irradiated, the subduploid cells The main proportion of the main passage, with the passage of times increasing gradually decreased, the polyploid cells in the late passage of the main, this trend may be related to the progress of cell transformation; (2) in the transformed cells, chromosome 15 long arm rearrangement (15q + Which may play an important role in the malignant transformation of RTE cells. (3) The frequency of Marker chromosomes M1, M2 and M3 increased gradually with the increase of passage number, especially M1 and M2 were high in tumorigenic cells Frequency (> 90%), suggesting that may be related to the progress of cell transformation.