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目的本研究应用无修饰的单壁纳米碳管(naked single wall nanotube,NSWNT)对小鼠灌胃,探讨单壁纳米碳管(single wall nanotube,SWNT)的生物安全性。方法SWNT分别以50,100,150mg·kg-1剂量对小鼠进行灌胃给药,连续给药10d后,于末次给药30min后,小鼠眼球取血及小鼠内脏采集,进行血液生化检查及组织切片的光学显微镜、透射电镜检查。结果SWNT可以导致丙氨酸氨基转移酶,天冬氨酸氨基转移酶,乳酸脱氢酶和肌酸激酶升高。光镜观察组织切片发现肝脏,肾脏,肺脏及胃肠道有显著的出血,充血。结论①SWNT可以导致肝脏和肾脏损伤;②SWNT可被胃肠道吸收;③由于脑组织未被NSWNT损伤,推测NSWNT可能不能通过血脑屏障。
Objective To study the biosafety of single-wall carbon nanotubes (SWNTs) in mice by using unmodified naked single wall nanotubes (NSWNTs). Methods SWNT were administered intragastrically to mice at dose of 50, 100 and 150 mg · kg-1, respectively. After continuous administration for 10 days, 30 min after the last administration, blood was collected from the eyeball and the viscera of mice were collected for blood biochemical examination and tissue Slices of light microscopy, transmission electron microscopy. Results SWNTs resulted in elevated alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and creatine kinase. Light microscopy of tissue sections found liver, kidney, lung and gastrointestinal tract significant bleeding, congestion. Conclusion ①SWNT can cause liver and kidney damage; ②SWNT can be absorbed by the gastrointestinal tract; ③Non-NTNT may not be able to cross the blood-brain barrier because brain tissue is not damaged by NSWNT.