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新生儿时期接受氧疗虽可挽救患儿的生命,但也可带来许多高氧相关的损伤。目的研究高氧对新生大鼠肠黏膜sIgA及肠道分泌片表达的影响。方法建立高氧动物模型,采用Elisa直接检测法检测sIgA,并采用Western blot方法观察检测肠道分泌片蛋白表达量的变化。结果高氧组新生大鼠肠黏液sIgA较空气组明显升高(P<0.01),肠道分泌片蛋白表达量与空气组相比明显增加(P<0.01)。结论持续高氧作用下,新生大鼠肠黏液分泌SIgA及肠道分泌片均增多,增强肠道抵御细菌、毒素的能力以及肠道的免疫功能,以保持肠道粘膜的完整性。
Oxygen therapy during the neonatal period can save the child’s life, but it can also cause many oxygen-related injuries. Objective To investigate the effect of hyperoxia on the expression of sIgA and gut secretion in intestinal mucosa of neonatal rats. Methods The animal model of hyperoxia was established. The sIgA was detected by Elisa direct assay and the protein expression of intestinal secretions was detected by Western blot. Results Compared with air group, the sIgA of intestinal mucus in neonatal rats in hyperoxia group was significantly increased (P <0.01), and the protein expression of intestinal secretions was significantly increased compared with air group (P <0.01). CONCLUSIONS: Under continuous hyperoxia, the secretion of SIgA and gut secretion in neonatal rats increased, which enhanced the ability of the gut to resist bacteria and toxins and the immune function of gut to maintain the integrity of intestinal mucosa.