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目的 :探讨转化生长因子 (TGF) α和表皮生长因子受体 (EGFR)在培养前后胎儿卵巢中的表达。方法 :收集 2 0例胎龄为 16～ 40周的水囊引产或各种原因死亡的胎儿卵巢标本 ,采用免疫组织化学方法 ,测定TGF α和EGFR蛋白在人胎儿卵巢组织中的表达。结果 :TGF α、EGFR在所有胎儿卵巢卵母细胞、部分卵泡细胞及基质细胞呈阳性表达 ,在卵母细胞的表达均强于卵泡细胞与基质细胞。胎龄与TGF α、EGFR的表达无相关关系 (P >0 .0 5 ) ,组织培养后卵母细胞TGF α、EGFR的表达强度均弱于培养前 (P <0 .0 5 )。结论 :胎儿卵巢存在局部调节因子。组织培养后胎儿卵巢TGF α与EGFR的表达与卵泡生长发育是一致的。TGF α与EGFR以自分泌旁 /分泌的方式协同参与胎儿卵母细胞的生长。
Objective: To investigate the expression of transforming growth factor (TGF) α and epidermal growth factor receptor (EGFR) in fetal ovary before and after culture. Methods: Twenty fetus ovarian samples were collected from gestational age of 16 to 40 weeks and induced by various causes of death. Immunohistochemistry was used to determine the expression of TGFα and EGFR protein in human fetal ovarian tissue. Results: TGFα and EGFR were positive in all fetal ovarian oocytes, some follicular cells and stromal cells, and oocytes were more expressed in follicular cells and stromal cells. The gestational age had no correlation with the expression of TGFα and EGFR (P> 0.05). The expression of TGFα and EGFR in oocytes after culture was weaker than that before culture (P <0.05). Conclusion: Fetal ovaries have local regulatory factors. Tissue culture fetal ovarian TGFα and EGFR expression and follicular growth and development are consistent. TGFα and EGFR synergistically participate in the growth of fetal oocytes in an autocrine, paracrine manner.