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利用聚合酶链式反应 (PCR)技术 ,体外扩增大豆花叶病毒 (SMV)的外壳蛋白基因 ,以建立检测SMV的新技术。从提纯的SMV病毒粒体中抽取RNA ,经反转录合成cDNA第一链 ,以此为模板 ,进行PCR扩增 ,组建了RT -PCR检测SMV的试剂盒。
The coat protein gene of soybean mosaic virus (SMV) was amplified by polymerase chain reaction (PCR) in vitro to establish a new technique for detecting SMV. RNA was extracted from the purified SMV virus mitochondria and the first strand of cDNA was synthesized by reverse transcriptase. The kit was used for PCR amplification, and a kit for detecting SMV by RT-PCR was established.