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成熟B细胞具有Ig重链和轻链的基因重排,而大多数非B细胞来源的造血细胞仍维持在胚系结构.这种Ig基因重排可作为一个敏感的特异指标,即使小于5%克隆性细胞群,也可证明其克隆性.淋巴瘤为恶性克隆起源的血液病,Ig基因的重排测定可以帮助其分型和预示早期复发.本文用Southern印迹技术分析人9 种恶性淋巴瘤细胞系,并于正常胚系DNA比较.这9种恶性淋巴瘤细胞系由美国国立癌症研究所提供,均为B细胞恶性淋巴瘤.实验结果表明,正常胚系DNA lgCλ基因表现为14Kb,8Kb,5Kb片断,SU-DHL-2细胞系表现为重排,比正常胚系细胞多出一个 4Kb片断,NU—DHL—1细胞系亦为重排,分别为13.5Kb,7Kb,4.9Kb和3Kb.Raji细胞系亦有3Kb重排,SB细胞系表现为13,5Kb,7Kb,4.7kb重排,Daudi细胞系亦是如此.8392细胞系缺失5Kb片断,仅SU-DHL-4,SU-DHL-5,PA-3细胞系维持胚系结构.每种细胞系个体的特异性重排是其恶性克隆起源的基因标志,这对于了解B淋巴细胞肿瘤生成,发展,演变的机制有重要意义.B 淋巴细胞起源的恶性肿瘤中,Cλ基因的重 排为20%～75%,而T淋巴细胞起源的恶性肿瘤中,Cλ基因的重排为0%.在髓系起源的细胞中亦无Cλ基因的重排,因此Cλ基因的重排可鉴别T,B细胞的起源和淋,粒细胞的起源.因为每种恶性克隆均有自己的Cλ基因的重排方式,这种个
Mature B cells have a genetic rearrangement of the Ig heavy and light chains, whereas most non-B cell-derived hematopoietic cells remain in the germline structure. This Ig gene rearrangement can be used as a sensitive specific indicator, even if less than 5% Clonal cell population can also prove its clonality. Lymphoma is a hematological disease of malignant clonal origin. The rearrangement of Ig gene can help in its classification and predict early recurrence. This paper analyzes 9 malignant lymphomas in human by Southern blot technique. The cell lines were compared with normal germline DNA. The 9 malignant lymphoma cell lines were provided by the National Cancer Institute of the United States and were all B cell lymphomas. The experimental results showed that the normal germline DNA lgCλ gene showed 14Kb, 8Kb. , 5Kb fragment, SU-DHL-2 cell line showed rearrangement, one more 4Kb fragment than normal germ line cell, NU-DHL-1 cell line was also rearranged, 13.5Kb, 7Kb, 4.9Kb and 3Kb respectively. The .Raji cell line also has a 3Kb rearrangement, and the SB cell line shows a 13,5 Kb, 7 Kb, 4.7 kb rearrangement, as does the Daudi cell line. The 8392 cell line lacks a 5 Kb segment, only SU-DHL-4, SU-DHL. -5, PA-3 cell lines were maintained germline configuration. each cell line is a subject-specific rearrangement malignant clonal origin Gene markers, which are important for understanding the mechanism of B lymphocyte tumorigenesis, development, and evolution. B. Malignant tumors of lymphocyte origin, rearrangement of Cλ genes is 20% to 75%, and malignant tumors of T lymphocyte origin The rearrangement of the Cλ gene is 0%. There is also no rearrangement of the Cλ gene in cells of myeloid origin. Therefore, the rearrangement of the Cλ gene can identify the origin of T and B cells and the origin of lymphocytes and granulocytes. The malignant clones all have their own rearrangement of Cλ genes.