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利用某些抗生素的内吸性 ,筛选出可使黑松幼苗组织抑制B61 9菌的抗生素溶液浓度 ;进行了抗生素对松材线虫病影响的试验 ,在非无菌条件下 ,用松材线虫对幼苗接种 ,结果表明用抗生素溶液培养的幼苗未发生萎蔫 ,而用无菌水培养的幼苗均出现萎蔫病状 ;同时试验后检查抗生素溶液培养的幼苗 ,发现其组织内均有活线虫存在。这一结果不仅进一步说明了松材线虫病是一种由松材线虫和细菌共同侵染引起的复合侵染病害 ,而且首次用试验证明了通过控制致病细菌的方法可以控制松材线虫病。该结果为开发防治松材线虫病及早期诊断的新方法、培育抗松材线虫病的转基因松树新品种、对松材线虫病致病机理的深入研究奠定了理论基础
The antibiotic concentration of antibiotic B61 9 was screened out by the internal absorption of some antibiotics. The effect of antibiotics on pine wilt disease was tested. Under non-sterile conditions, Seedlings were inoculated. The results showed that the seedlings cultured in antibiotic solution did not wilting, while the seedlings cultured in sterile water showed wilting symptoms. After the test, the seedlings of the antibiotic solution were examined and found to have live worms in their tissues. This result not only further demonstrates that pine wilt disease is a composite infestation caused by co-infection of pine wood nematode and bacteria, and for the first time demonstrates that pine wilt disease can be controlled by controlling pathogenic bacteria. The results provide a theoretical basis for the development of new methods of preventing and controlling pine wood nematode disease and early diagnosis, breeding new varieties of transgenic pine wood against pine nematode disease, and further studying the pathogenesis of pine wood nematode disease