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为探明木麻黄对自毒胁迫的抗性机理,进而为解决木麻黄连栽障碍提供实验依据,以同一基因型的‘惠安1号’木麻黄(Casuarina equisetifolia L.)水培幼苗为材料,研究了木麻黄两种化感物质槲皮黄素-3-α-阿拉伯糖苷(化感物质A)和槲皮黄素-3-β-葡萄糖苷(化感物质B)0(CK)、12.5、25、50、100、200、400 mg L-1浓度模拟胁迫对木麻黄幼苗小枝叶绿素荧光特性的影响,并用模糊数学的隶属函数法对幼苗的抗化感胁迫性进行综合评价.结果显示:在轻度(12.5、25、50 mg L-1)胁迫下木麻黄F0、Fm、Fv/F0和Fv/Fm变化不大,Fv/Fm仍保持0.78左右,光合生理正常进行.随着化感物质胁迫浓度的升高(100、200、400 mg L-1),木麻黄幼苗小枝的F0、qN、Drate、LPAR显著升高(P<0.05),Fm、Fv/Fm、Fv/F0、Prate、ΦPSⅡ、qP显著下降(P<0.05),表明化感物质胁迫对木麻黄幼苗小枝PSⅡ反应中心造成了破坏,光电子传递速率降低,热耗散途径受阻,影响了激发能在PSⅠ和PSⅡ的平衡分配,光合机构受损.而不同化感物质胁迫下木麻黄幼苗小枝叶绿素荧光参数随胁迫程度的变幅不同,木麻黄对化感物质A的抗性强于对化感物质B.
In order to explore the resistance mechanism of Casuarina equisetifolia to autotoxic stress and to provide the experimental basis for solving the problem of Casuarina equisetifolia cultivation, using the hydroponic seedlings of the same genotype “Casuarina equisetifolia L.” The effects of quercetin-3-α-arabinoside (Allelochemical A) and quercetin-3-β-glucoside (Allergen B) 0 , 50, 100, 200, 400 mg L-1 concentration stress on the branchlet chlorophyll fluorescence characteristics of Casuarina equisetifolia seedlings and using fuzzy mathematics membership function method to evaluate the resistance of seedlings to stress.The results showed that: The Fv, Fm, Fv / F0 and Fv / Fm of Casuarina equisetifolia under the moderate (12.5, 25 and 50 mg L-1) stress did not change much while the Fv / Fm remained about 0.78 and the photosynthetic physiology was normal. F0, qN, Drate and LPAR were significantly increased (P <0.05), Fm, Fv / Fm, Fv / F0, Prate, ΦPSⅡ , QP decreased significantly (P <0.05), indicating that allelopathic stress caused damage to PSⅡ reaction center of branchlet of Casuarina equisetifolia seedlings, decreased photoelectron transfer rate, heat dissipation Diameter blocked, affecting the excitation energy in the balance of PS Ⅰ and PS Ⅱ distribution, photosynthetic machinery damage.Under different allelochemicals Stress Caskiura different of the twig chlorophyll fluorescence parameters with different stress amplitude, Casuarina allelochemicals A Resistant to allergens than substance B.