论文部分内容阅读
目的 :探讨铅对大鼠海马神经元 Ca2 +浓度的影响及其与长时程增强 (L TP)的关系。方法 :断乳后 Wistar大鼠自由饮用不同浓度醋酸铅溶液 (0 .0 15 % ,0 .10 % ,0 .15 % ) ,建立慢性染铅动物模型。高频刺激 (HFS)海马区诱发长时程变化后 ,分离海马神经元 ,测定 [Ca2 + ]i。结果 :各染铅组大鼠海马神经元 [Ca2 + ]i与对照组比较均明显增高(P <0 .0 5 ) ;血铅浓度 (3.2 8± 0 .88) μm ol/ L 以上时海马 L TP产生率下降 ,三染铅组 PS幅值均降低 ,L TP阴性组 [Ca2 + ]i 明显高于 L TP阳性组 (P <0 .0 5 )。结论 :慢性染铅可使 HFS后大鼠海马神经元 [Ca2 + ]i 升高 ,损害海马区 L TP的在体诱导 ;铅致 L TP发生率下降与海马神经元 [Ca2 + ]i 增高有关
Objective: To investigate the effect of lead on Ca2 + concentration in hippocampal neurons of rats and its relationship with long-term potentiation (LTP). Methods: Wistar rats were fed with different concentrations of lead acetate solution (0. 15%, 0. 10%, 0. 15%) freely after weaning to establish animal models of chronic lead exposure. After long-term changes induced by high-frequency stimulation (HFS) in hippocampus, hippocampal neurons were isolated and [Ca2 +] i was measured. Results: The levels of [Ca2 +] i in hippocampus of hippocampus of all the lead-exposed groups were significantly higher than those of the control group (P <0.05); hippocampus of the hippocampus of the lead-exposed group was (3.2 8 ± 0.88) μmol / L L TP decreased, and the amplitude of PS in the three lead-treated groups decreased. The [Ca2 +] i in the LTP-negative group was significantly higher than that in the LTP-positive group (P <0.05). CONCLUSION: Chronic lead exposure can increase the [Ca2 +] i of hippocampal neurons in hippocampus of rats after HFS and impair the in vivo induction of LTP in hippocampus. The decrease of LTP induced by lead is related to the increase of [Ca2 +] i in hippocampal neurons