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采用逆转录ＰＣＲ技术自ＳＤ大鼠脾细胞中克隆出全长１１２５个ｂｐ的编码主要组织相容性抗原Ⅰ类分子（ＭＨＣ－Ⅰ）ｃＤＮＡ，经限制性内切酶图谱分析证实后，定向插入表达载体ｐＢＶ２２０，并筛选出带有插入片断的阳性克隆，为研究在原核或真核表达系统中表达及其在抗原识别、免疫应答中的作用奠定了基础。
Reverse transcriptase-PCR was used to clone the cDNA encoding the major histocompatibility class I antigen (MHC-I) of 1125 bp in length from the spleen cells of SD rats. After confirmed by restriction endonuclease mapping, The expression vector pBV220 was screened and positive clones with inserted fragments were screened, which laid the foundation for studying the expression in prokaryotic or eukaryotic expression system and its role in antigen recognition and immune response.