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北京地区水稻白叶枯细菌噬菌体在胨蔗糖(PBS)平板培养基上其溶菌斑有两种类型:Ⅰ型为大斑型PK,直径范围约4毫米;Ⅱ型为小斑型(PSS)直径约1毫米。电子显微镜形态观察结果:两者均呈蝌蚪形,具六角形头部,但小斑型PSS 为收缩性短尾,大斑型PK 为非收缩性的长尾。Ⅰ型PK 头部大小为60×59nm,尾部为133×12nm;Ⅱ型PSS 头部为70×59nm,尾部短为95×16nm。研究比较了两种噬菌体抗血清,大斑型抗血清K 值为14,小班型K 值为300。通过溶斑大小、电镜形态和血清学交叉反应测定的结果,认为北京地区水稻白叶枯细菌的噬菌体有两种不同的类型。
There are two types of bacteriolytic bacteriolytic bacteriophages in Beijing peanut bacteriophage on peptone sucrose (PBS) medium: type I is large plaque type PK with a diameter of about 4 mm; type II is small plaque type (PSS) diameter About 1 mm. Electron microscopy Morphological observations: Both showed a tadpole shape with a hexagonal head, but the small plaque type PSS was a contracting short tail, and the large plaque type PK was a non-contracting long tail. Type Ⅰ PK head size of 60 × 59nm, the tail of 133 × 12nm; Ⅱ type PSS head of 70 × 59nm, tail short as 95 × 16nm. Two phage antisera were compared, the K value of the macrophage type antiserum was 14, and the K value of the small class was 300. According to the results of lysing size, electron microscopy and serological cross-reactivity, there are two different types of bacteriophages in rice bacterial blight in Beijing.