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目的探讨乙醇预处理对肝脏缺血再灌流损伤的影响以及与热休克蛋白70诱导的关系。方法雄性成年Wistar大鼠232只,胃饲乙醇浓度40%,剂量为5 g/kg体重,随机分为5组:正常对照组(N组)、胃饲乙醇组(E组)、缺血组(IR组)、生理盐水预处理组(NPC组)、胃饲乙醇预处理组(EPC组)。动物手术采用门静脉转流下的肝脏缺血模式,肝门阻断时限为90 min,于再灌流0、13、、6、12、24、72 h活杀留取血液及肝脏标本。结果EPC组3、6 h血清ALT(1 230.88±132.50、888.88±126.67)IU/L、AST(1 866.38±61.77)(、1 433.88±42.74)IU/L均明显低于IR组及NPC组,肝脏病理改变较轻,而肝组织HSP70含量高于后者。结论乙醇预处理可以减轻大鼠肝脏90 min的缺血再灌流损伤,其肝脏保护作用与肝组织HSP70升高相一致,HSP70可能是其发挥肝脏保护的物质基础之一。
Objective To investigate the effect of ethanol preconditioning on hepatic ischemia-reperfusion injury and its relationship with heat shock protein 70 induction. Methods Male adult Wistar rats, 232, were fed with 40% ethanol and 5 g / kg body weight. They were randomly divided into 5 groups: normal control group (N group), gastric fed group (E group), ischemia group (IR group), saline preconditioning group (NPC group), gastric feeding ethanol pretreatment group (EPC group). Animal models of hepatic ischemia with portal vein bypass were used, and the portal vein occlusion time was 90 min. Blood and liver samples were collected at 0, 13, 6, 12, 24 and 72 h after reperfusion. Results The levels of serum ALT (1 230.88 ± 132.50, 888.88 ± 126.67) IU / L and AST (1 866.38 ± 61.77) (1 433.88 ± 42.74) IU / L in EPC group were significantly lower than those in IR group and NPC group Pathological changes in liver lighter, and liver tissue HSP70 content higher than the latter. Conclusions Ethanol preconditioning can reduce ischemia-reperfusion injury in the liver of rats for 90 min. The hepatic protective effect is consistent with the increase of hepatic HSP70. HSP70 may be one of the material bases for liver protection.